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JoVE Journal
Genetics

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Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
 

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Article DOI: 10.3791/57920
September 15th, 2018

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Summary September 15th, 2018

micro-RNAs (miRNAs) are short and highly homologous RNA sequences, serving as post-transcriptional regulators of messenger RNAs (mRNAs). Current miRNA detection methods vary in sensitivity and specificity. We describe a protocol that combines in situ hybridization and immunostaining for concurrent detection of miRNA and protein molecules on mouse heart tissue sections.

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