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Immunology and Infection

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Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Article DOI: 10.3791/57941-v • 09:12 min • October 17th, 2018
October 17th, 2018 •
1,2, 1,2, 3, 4, *1,2, *1,2
1Department of Surgery, Vascular Biology Research Centre, Westmead Hospital, 2Westmead Clinical School, Department of Surgery, The University of Sydney, 3Westmead Research Hub, Westmead Institute for Medical Research, 4Institute for Clinical Pathology and Medical Research, Westmead Hospital
* These authors contributed equally

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Summary
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Here we present a protocol for characterizing monocyte subsets by whole blood flow cytometry. This includes outlining how to gate the subsets and assess their expression of surface markers and giving an example of the assessment of the expression of M1 (inflammatory) and M2 markers (anti-inflammatory).

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Whole Blood Flow Cytometry Analysis Monocyte Subsets Markers Disease States Standardized Gating Method Blood Samples White Blood Cell Count Dilution Master Mix Antibodies T Cells B Cells Neutrophils Natural Killer Cells Lysis Solution Fixing Solution
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