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JoVE Journal
Biochemistry

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Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
 

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+

Article DOI: 10.3791/57979 08:26 min June 25th, 2018
June 25th, 2018

Chapters

Summary

We describe a simple and rapid method for the preparation and analysis of N-glycans from different cultivars of radish (Raphanus sativus).

Transcript

Tags

N-glycans Raphanus Sativus Plant Glycoscience PNGase H Enzymatic Release Derivatization Reactions 2-aminobenzamide Glycomics And Glycan Bioengineering Research Center Nanjing Agricultural University Proteins Isolation Fresh Radish Centrifuge Tube Trichloroacetic Acid Solution Deionized Water
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