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JoVE Journal
Genetics

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High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping
 

High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping

Article doi: 10.3791/58024
June 29th, 2018

Summary June 29th, 2018

Zebrafish have been used as reliable genetic model organisms in biomedical research, especially with the advent of gene-editing technologies. When larval phenotypes are expected, DNA extraction and genotype identification can be challenging. Here, we describe an efficient genotyping procedure for zebrafish larvae, by tail clipping, as early as 72-h post-fertilization.

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