JoVE Journal
Developmental Biology
Developmental Biology
A subscription to JoVE is required to view this content.
Chapters
Summary
In this protocol, we aim to describe a reproducible method for combining dissociated human pluripotent stem cell derived neurons and astrocytes together into 3D sphere cocultures, maintaining these spheres in free floating conditions, and subsequently measuring synaptic circuit activity of the spheres with immunoanalysis and multielectrode array recordings.