Journal
/
Biology
/
Anaerobic Growth and Maintenance of Mammalian Cell Lines
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Anaerobic Growth and Maintenance of Mammalian Cell Lines

Anaerobic Growth and Maintenance of Mammalian Cell Lines

DOI:
10.3791/58049-v

•

07:15 min

•

July 21, 2018

•

, , ,
1Department of Microbiology and Immunology,Midwestern University

Chapters

  • 00:04Title
  • 01:11Prepare Media for Anaerobic Culture of Various Mammalian Cell Lines
  • 02:14Anoxic Cultivation of Various Mammalian Cell Lines
  • 04:33Assessment of Phenotypic Cell Differentiation by Microscopy of Anaerobically Cultured Cells
  • 05:10Results: Cell Viability for Long-term Cultivation Dependent on Initial Seeding Confluence
  • 05:57Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a new method that enables the anaerobic long-term cultivation of established cell lines. The maximum survival time that was tested is 17 days. This method is suitable for the testing of cytotoxic agents and exploring the physiology of anoxically replicating cells.

Tags

Keywords: Anaerobic GrowthMammalian Cell LinesAnoxic CultivationOxygen ToxicityAnaerobic RespirationMetabolismStem Cell CultivationOxygen ContaminationNitriteDMEMVacuumAnaerobic ChamberOxygen ProbeTrypsinCell CountingCell Viability

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58049?article_type=v

Passaging HuES Human Embryonic Stem Cell-lines with Trypsin.
check_url/58049?article_type=v

Preparing T Cell Growth Factor from Rat Splenocytes
check_url/58049?article_type=v

Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth
check_url/58049?article_type=v

MISSION esiRNA for RNAi Screening in Mammalian Cells
check_url/58049?article_type=v

Analysis of Cell Cycle Position in Mammalian Cells
check_url/58049?article_type=v

Determination of Mammalian Cell Counts, Cell Size and Cell Health Using the Moxi Z Mini Automated Cell Counter
check_url/58049?article_type=v

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
check_url/58049?article_type=v

Measurement of Heme Synthesis Levels in Mammalian Cells
check_url/58049?article_type=v

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
check_url/58049?article_type=v

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

Read Article