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Biology

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Anaerobic Growth and Maintenance of Mammalian Cell Lines
 

Anaerobic Growth and Maintenance of Mammalian Cell Lines

Article doi: 10.3791/58049
July 21st, 2018

Summary July 21st, 2018

Here, we describe a new method that enables the anaerobic long-term cultivation of established cell lines. The maximum survival time that was tested is 17 days. This method is suitable for the testing of cytotoxic agents and exploring the physiology of anoxically replicating cells.

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