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Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
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Biochemistry
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JoVE Journal Biochemistry
Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis

Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis

DOI:
10.3791/58076-v

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08:07 min

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September 11, 2018

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, , ,
1Department of Adaptive Biotechnologies, Global Change Research Institute,Czech Academy of Sciences, 2Department of Plant Physiology, Faculty of Science,Masaryk University, 3Laboratory of Intracellular Regulation, Institute of Plant Physiology,Russian Academy of Sciences

Chapters

  • 00:00Title
  • 01:10Samples Preparation
  • 02:34Cells Homogenization and Pigment Extraction
  • 03:47Phycobiliproteins Quantification
  • 04:47Representative Results
  • 07:30Conclusion

Summary

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Here, we present a protocol to quantitatively determine phycobiliprotein content in the cyanobacterium Synechocystis using a spectrophotometric method. The extraction procedure was also successfully applied to other cyanobacteria and algae strains; however, due to variations in pigment absorption spectra, it is necessary to test the spectrophotometric equations for each strain individually.

Tags

Keywords: Spectrophotometric DeterminationPhycobiliproteinsCyanobacteriumSynechocystisPhycocyaninAllophycocyaninPigment-protein ComplexesLight-harvesting AntennaeCyanobacteria ProductivityExtractionFreeze-dryingHomogenization

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