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Biochemistry

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JoVE Journal Biochemistry
Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion
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Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Article DOI: 10.3791/58079-v • 07:54 min • August 29th, 2018
August 29th, 2018 •
1,2,3, 1, 1, 1, 1,2, 1
1The Smidt Heart Institute, Cedars-Sinai Medical Center, 2Advanced Clinical Biosystems Research Institute, Cedars-Sinai Medical Center, 3Institute of Analytical Chemistry of the Czech Academy of Sciences

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Here we present a protocol to perform polysome profiling on the isolated perfused mouse heart. We describe methods for heart perfusion, polysome profiling, and analysis of the polysome fractions with respect to mRNAs, miRNAs, and the polysome proteome.

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Dynamic Proteomic Analysis MiRNA Analysis Polysomes Langendorff Perfusion Protein Synthesis Translation Process MRNAs MicroRNAs Stress Response Organ-specific Response Gradient Mixer Sucrose Gradient Solution Ultracentrifuge Tube Homogenize Tissue Lysis Buffer Protein Determination
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