JoVE Journal
Biochemistry
Biochemistry
A subscription to JoVE is required to view this content.
Chapters
Summary
Here, we present a protocol to produce house fly carboxylesterase proteins in vitro with a baculovirus mediated insect cell expression system and later functionally characterize their roles in metabolizing permethrin, thereby, conferring pyrethroid resistance by conducting cell-based MTT assay and in vitro metabolic studies.