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JoVE Journal
Bioengineering

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Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation
 

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

Article doi: 10.3791/58123
November 26th, 2018

Summary November 26th, 2018

This method enables decellularization of a complex solid organ using a simple protocol based on osmotic shock and perfusion of ionic detergent with minimal organ matrix disruption. It comprises a novel decellularization technique for human hearts inside a pressurized pouch with real-time monitoring of flow dynamics and cellular debris outflow.

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