A High-performance Liquid Chromatography Measurement of Kynurenine and Kynurenic Acid: Relating Biochemistry to Cognition and Sleep in Rats

JoVE Journal
Behavior

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JoVE Journal Behavior

DOI:

10.3791/58129-v

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07:04 min

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August 19, 2018

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Annalisa M. Baratta, Shaun S. Viechweg, Jessica A. Mong, Ana Pocivavsek

¹Maryland Psychiatric Research Center, Department of Psychiatry,University of Maryland School of Medicine, ²Department of Pharmacology,University of Maryland School of Medicine

Chapters

00:04Title
00:40Sample Preparation
02:10High-performance Liquid Chromatography (HPLC) Kynurenine Measurement
05:17Results: Effects of Kynurenine Elevation on Hippocampal-dependent Memory and Sleep-wake Architecture
06:44Conclusion

Summary

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Alterations in the kynurenine pathway (KP) neuroactive metabolites are implicated in psychiatric illnesses. Investigating the functional outcomes of an altered kynurenine pathway metabolism in vivo in rodents may help elucidate novel therapeutic approaches. The current protocol combines biochemical and behavioral approaches to investigate the impact of an acute kynurenine challenge in rats.

A High-performance Liquid Chromatography Measurement of Kynurenine and Kynurenic Acid: Relating Biochemistry to Cognition and Sleep in Rats

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