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In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers

In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers

DOI:
10.3791/58139-v

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08:10 min

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July 28, 2018

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, ,
1Department of Cellular and Molecular Biophysics,Max Planck Institute of Biochemistry

Chapters

  • 00:04Title
  • 00:24Small Unilamellar Vesicle (SUV) Preparation
  • 01:20Cleaning Glass Coverslips
  • 02:13Chamber Assembly and Supported Lipid Bilayer (SLB) Formation
  • 04:15PDMS microstructures
  • 05:48Self-organization in PDMS Microstructures
  • 07:11Results: MinDE Self-Organization on Supported Lipid Bilayers
  • 07:44Conclusion

Summary

Automatic Translation

Automatic Translation

We provide a protocol for in vitro self-organization assays of MinD and MinE on a supported lipid bilayer in an open chamber. Additionally, we describe how to enclose the assay in lipid-clad PDMS microcompartments to mimic in vivo conditions by reaction confinement.

Tags

Keywords: In Vitro ReconstitutionSelf-organizing Protein PatternsSupported Lipid BilayersGeometric CuesPattern FormationMultilamellar VesiclesFreeze-thawLipid ExtruderSulfuric AcidHydrogen PeroxideCoverslipsSupported Lipid Bilayer FormationHeat Block

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