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Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
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JoVE Journal Medicine
Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses

DOI:
10.3791/58148-v

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11:17 min

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August 30, 2018

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, , , , ,
1Institute of Laboratory Medicine, University Hospital,LMU Munich

Chapters

  • 00:04Title
  • 01:05Preparation of the Calibrators, Quality Control Samples, and Internal Standards Mix
  • 02:29Buffer Preparation for Chromatography
  • 03:47Sample Cleanup and HPLC-MS/MS Analysis
  • 06:28Quality Assessment and Quantification
  • 08:14Results: Data Analysis and Quantification of Piperacillin
  • 09:44Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a tandem mass spectrometry-based protocol for the quantification of frequently used antibiotics in intensive care units, namely cefepime, meropenem, ciprofloxacin, moxifloxacin, linezolid, and piperacillin.

Tags

Keywords: AntibioticsTherapeutic Drug MonitoringMass SpectrometryHPLC-MS/MSIntensive CareCefepimeMeropenemCiprofloxacinMoxifloxacinLinezolidPiperacillinPharmacokineticsSerum CalibratorsQuality ControlsInternal StandardsMobile PhaseFormic AcidAmmonium Formate

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