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JoVE Journal
Medicine

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An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney
 

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney

Article DOI: 10.3791/58162
November 1st, 2018

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Summary November 1st, 2018

The main focus of this protocol is to efficiently isolate viable primary glomeruli cultures with minimal contaminants for use in a variety of downstream applications. The isolated glomeruli retain structural relationships between component cell types and can be cultured ex vivo for a short time.

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