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JoVE Journal
Medicine

This content is Open Access.

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney
 

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney

Article doi: 10.3791/58162
November 1st, 2018 Usage Statistics

Summary November 1st, 2018

The main focus of this protocol is to efficiently isolate viable primary glomeruli cultures with minimal contaminants for use in a variety of downstream applications. The isolated glomeruli retain structural relationships between component cell types and can be cultured ex vivo for a short time.

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