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Journal / Immunology and Infection / PCR quantitativa del batteriofago T7 da Biopanning
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Immunology and Infection

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JoVE Journal Immunology and Infection
PCR quantitativa del batteriofago T7 da Biopanning
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PCR quantitativa del batteriofago T7 da Biopanning

Article DOI: 10.3791/58165-v • 05:42 min • September 27th, 2018
September 27th, 2018 •
1, 1, 1, 1, 1
1Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy, University of Texas at Austin

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Un tempo, accurato e riproducibile efficiente quantitativa PCR (qPCR) metodo per enumerare fago T7 è descritto qui. Il protocollo descrive chiaramente la preparazione di DNA genomico dei fagi, preparazione di reazione di PCR, qPCR ciclismo condizioni e analisi dei dati di qPCR.

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Immunologia e infezione problema 139 fago T7 phage DNA genomic qPCR saggio della placca limite di rilevazione gamma di quantificazione
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