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JoVE Journal
Immunology and Infection

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Quantitative PCR of T7 Bacteriophage from Biopanning
 

Quantitative PCR of T7 Bacteriophage from Biopanning

Article doi: 10.3791/58165
September 27th, 2018

Summary September 27th, 2018

A reproducible, accurate, and time efficient quantitative PCR (qPCR) method to enumerate T7 bacteriophage is described here. The protocol clearly describes phage genomic DNA preparation, PCR reaction preparation, qPCR cycling conditions, and qPCR data analysis.

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