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JoVE Journal
Immunology and Infection

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Quantitative PCR of T7 Bacteriophage from Biopanning

Quantitative PCR of T7 Bacteriophage from Biopanning

Article DOI: 10.3791/58165 05:42 min September 27th, 2018
September 27th, 2018



A reproducible, accurate, and time efficient quantitative PCR (qPCR) method to enumerate T7 bacteriophage is described here. The protocol clearly describes phage genomic DNA preparation, PCR reaction preparation, qPCR cycling conditions, and qPCR data analysis.



Quantitative PCR T7 Bacteriophage Biopanning Virology Microbiology Enumeration Diagnostics Therapeutics Time Efficient Accurate Quantification Phage Display Biopanning Experiments Primers Stock Concentration Reference T7 Phage DNA Serial Dilution Femtograms Per Microliter PCR Tubes Genome Copies Per Microliter Micro Centrifuge Tube DNase One Heated Dry Bath
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