Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Covalent Immobilization of Proteins for the…
JoVE Journal
Biochemistry

This content is Open Access.

JoVE Journal Biochemistry
Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster
Journal (Biochemistry)
A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster
Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS
Journal (Biochemistry)
Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS
 

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Article DOI: 10.3791/58167-v • 11:13 min • August 20th, 2018
August 20th, 2018 •
1,2,3, 2, 1,3, 3, 2, *4, *1,3
1Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, 2FG Protein Biochemistry & Cellular Microbiology, Munich University of Applied Sciences, 3Center for Nano Science, Ludwig-Maximilians-Universität München, 4Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie, University Medical Center Göttingen
* These authors contributed equally

Chapters

Summary
Automatic Translation

This protocol describes the covalent immobilization of proteins with a heterobifunctional silane coupling agent to silicon-oxide surfaces designed for the atomic force microscopy based single molecule force spectroscopy which is exemplified by the interaction of RrgA (pilus-1 tip adhesin of S. pneumoniae) with fibronectin.

Tags

Covalent Immobilization Proteins Single Molecule Force Spectroscopy Biophysical Field Protein Interactions Biomolecules Atomic Force Microscope Cantilever Probe Acid Resistant Gloves Safety Goggles Laboratory Coat Isopropanol Lint-free Precision Wipes Glass Slides Hydrochloric Acid Ultrasonic Bath Silicon Nitride AFM Cantilever Probes UV Light-impermeable Chamber Ultraviolet Light Staining Jar Doubly Distilled Water Ethoxy Silane Polyethylene Glycol Acid
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter