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Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee
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Biochemistry
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JoVE Journal Biochemistry
Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee

Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee

DOI:
10.3791/58173-v

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08:30 min

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September 14, 2018

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, ,
1Bioproduction Research Institute,National Institute of Advanced Industrial Science and Technology, 2Department of Biological Sciences, Graduate School of Science,The University of Tokyo

Chapters

  • 00:04Title
  • 00:39Dissection of the Honeybee Brain
  • 02:59Preparation of Brain Homogenates
  • 04:21PLC Reaction in Brain Homogenates
  • 05:28Detection of Fluorescence Resulting from PLC Activity
  • 05:56Results: PLC Activities With and Without PLC Inhibitors in the Mushroom Bodies and Other Brain Regions
  • 07:25Conclusion

Summary

Automatic Translation

Automatic Translation

To test the inhibitory effects of pharmacologic agents on phospholipase C (PLC) in different regions of the honeybee brain, we present a biochemical assay to measure PLC activity in those regions. This assay could be useful for comparing PLC activity among tissues, as well as among bees exhibiting different behaviors.

Tags

Phospholipase C ActivityBrain HomogenateHoneybeeNeuroscienceDissectionHomogenizationEnzyme Assay

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