Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Hyaluronic-Acid Based Hydrogels for 3-Dimensional …
JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content.

JoVE Journal Bioengineering
Hyaluronic-Acid Based Hydrogels for 3-Dimensional Culture of Patient-Derived Glioblastoma Cells
Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model
Journal (Bioengineering)
Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model
A Rapid Image-based Bacterial Virulence Assay Using Amoeba
Journal (Bioengineering)
A Rapid Image-based Bacterial Virulence Assay Using Amoeba
 

Hyaluronic-Acid Based Hydrogels for 3-Dimensional Culture of Patient-Derived Glioblastoma Cells

Article DOI: 10.3791/58176-v • 08:35 min • August 24th, 2018
August 24th, 2018 •
1, 1, 1, 2
1Department of Bioengineering, University of California, Los Angeles, 2Department of Bioengineering, Jonsson Comprehensive Cancer Center, Broad Stem Cell Research Center, Brain Research Institute, University of California, Los Angeles

Chapters

Summary
Automatic Translation

Here, we present a protocol for three-dimensional culture of patient-derived glioblastoma cells within orthogonally tunable biomaterials designed to mimic the brain matrix. This approach provides an in vitro, experimental platform that maintains many characteristics of in vivo glioblastoma cells typically lost in culture.

Tags

Hyaluronic-acid Hydrogels 3-dimensional Culture Patient-derived Glioblastoma Cells Extracellular Matrix Treatment Resistance Cell Culture Platforms Native Brain Silicone Rubber Molds Non-tissue Culture Treated Plate Pipette Tip Centrifugation Cell Dissociation Enzyme Complete Medium Cell Straining 70 Micrometer Filter Polyethylene Glycol Maleimide (PEG-Mal-RGD)
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter