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JoVE Journal
Biology

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Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy
 

Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy

Article doi: 10.3791/58206
October 11th, 2018

Summary October 11th, 2018

We present use of 2-photon microscopy to place a micropipette within Bowman's urinary space in mice, combining 2 foundational techniques of renal physiology. Use of 2-photon microscopy overcomes critical limitations of conventional microscopy for micropuncture renal physiology studies.

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