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Journal / Biology / Micropuncture of Bowman's Space in Mice…
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Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy
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Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy

Article DOI: 10.3791/58206-v • 07:37 min • October 11th, 2018
October 11th, 2018 •
1, 1, 2, 2, 2, 2, 1,3
1Anesthesiology & Perioperative Medicine, Oregon Health & Science University, 2Environmental and Biological Services Division, Pacific Northwest National Laboratory, 3Operative Care Division, Portland Veterans Affairs Medical Center

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Summary
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We present use of 2-photon microscopy to place a micropipette within Bowman's urinary space in mice, combining 2 foundational techniques of renal physiology. Use of 2-photon microscopy overcomes critical limitations of conventional microscopy for micropuncture renal physiology studies.

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Micropuncture Bowman's Space Two-photon Microscopy Single-nephron Physiology Glomerular Filtration Rate Systemic Proteins Metabolites Tubular Physiology Cortical Nephron Preparation Steps Ointment Tape Depilatory Cream Incision Peritoneum Kidney Stabilizer Form Cyanoacrylate Adhesive Head Plate Mounting Bars Agarose Solution
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