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Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells

Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells

DOI:
10.3791/58216-v

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07:48 min

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September 06, 2018

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, , , , ,
1U861, I-Stem, Association Française contre les Myopathies (AFM),Institut National de la Santé et de la Recherche Médicale (INSERM), 2U861, I-Stem, Association Française contre les Myopathies (AFM),Université Evry Val-d’Essonne (UEVE), 3I-Stem, Association Française contre les Myopathies (AFM),Centre pour L’Etude des Cellules Souches (CECS), 4Banque de tissus humain, Hôpital Saint Louis, Assistance Publique – Hôpitaux de Paris (AP-HP), 5Sorbonne Université, INSERM, CNRS, Institut de la Vision, F-75012

Chapters

  • 00:04Title
  • 00:36Human Amniotic Membrane (hAM) Thermolysin Treatment and Fixation
  • 02:27Retinal Pigment Epithelium (RPE) Cell Seeding
  • 03:33RPE Patch Transplantation Preparation
  • 05:58Results: Representative RPE-seeded hAM
  • 06:38Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method to engineer a retinal tissue composed of retinal pigment epithelial cells derived from human pluripotent stem cells cultured on top of human amniotic membranes and its preparation for grafting in animal models.

Tags

Keywords: Retinal Pigment EpitheliumHuman Embryonic Stem CellsEye ImplantationRPE CellsAmniotic MembraneNylon ScaffoldThermolysinCulture InsertCell SeedingCell Culture

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