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JoVE Journal
Biochemistry

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Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette
 

Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow

Article DOI: 10.3791/58228-v
January 7th, 2019

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Summary January 7th, 2019

Mechanosensitive ion channels are often studied in terms of fluid flow/shear force sensitivity with patch-clamp recording. However, depending on the experimental protocol, the outcome on fluid flow-regulations of ion channels can be erroneous. Here, we provide methods for preventing and correcting such errors with a theoretical basis.

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