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JoVE Journal
Immunology and Infection

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Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins
 

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

Article DOI: 10.3791/58237
June 14th, 2018

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Summary June 14th, 2018

Here, we present a protocol to genetically modify primary or expanded human natural killer (NK) cells using Cas9 Ribonucleoproteins (Cas9/RNPs). By using this protocol, we generated human NK cells deficient for transforming growth factor–b receptor 2 (TGFBR2) and hypoxanthine phosphoribosyltransferase 1 (HPRT1).

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