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August 25, 2018
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Here we present a next-generation sequencing protocol for 16S rRNA sequencing which enables identification and characterization of microbial communities within vectors. This method involves DNA extraction, amplification and barcoding of samples through PCR, sequencing on a flow-cell, and bioinformatics to match sequence data to phylogenetic information.
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Cite this Article
Couper, L., Swei, A. Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing. J. Vis. Exp. (138), e58239, doi:10.3791/58239 (2018).
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