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Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue

Article DOI: 10.3791/58278-v • 06:36 min • May 1st, 2019
May 1st, 2019 •
*1, *1, 2, 1, 1, 1
1Department of Medical Oncology, Lung Cancer and Gastrointestinal Unit, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, 2Hunan ClinicaI Research Center in Gynecologic Cancer, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University
* These authors contributed equally

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Summary
Automatic Translation

Here, we present a protocol of elastic staining to identify elastic fibers in pT3N0M0 gastric cancer tissues on formalin-fixed, paraffin-embedded sections. Subsequently, we describe a method to determine whether tumor cells invade beyond the elastic lamina.

Tags

Elastic Staining Paraffin-embedded Slides PT3N0M0 Gastric Cancer Tumor Invasion Elastic Lamina Erosion Prognostic Picture Pathologist Evaluation Convenient Approach Accurate Identification Pathologic Test Stage Diagnosis TNM Stage System Lung Cancer Colo-rectal Cancer Xylene Immersion Ethanol Immersion Tissue Section Oxidation
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