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Journal / Developmental Biology / Analyse av Epididymal proteinsyntese og sekresjon
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JoVE Journal Developmental Biology
Analyse av Epididymal proteinsyntese og sekresjon
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Analyse av Epididymal proteinsyntese og sekresjon

Article DOI: 10.3791/58308-v • 10:23 min • August 25th, 2018
August 25th, 2018 •
1,2, 3, 1,2, 2,4, 1,2
1Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, 2Hunter Medical Research Institute, 3Department of Physiology, Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, 4School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle

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Her rapporterer vi immunofluorescence lokalisering av dynamin å illustrere protokollene for påvisning av proteiner i parafin-innebygd mus epididymal inndelinger og de av en udødeliggjort epididymal celle linje (mECap18). Vi beskriver også protokollene for isolering av sekretoriske proteiner fra både epididymal væske og betinget cellen media.

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Utviklingspsykologi biologi problemet 138 Dynamin bitestikkel epididymosome exosome immunofluorescence protein sekresjon sperm sperm modning
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