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Detection and Isolation of Apoptotic Bodies to High Purity
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Biochemistry
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JoVE Journal Biochemistry
Detection and Isolation of Apoptotic Bodies to High Purity

Detection and Isolation of Apoptotic Bodies to High Purity

DOI:
10.3791/58317-v

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12:17 min

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August 12, 2018

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, ,
1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science,La Trobe University

Chapters

  • 00:00Title
  • 00:53Induction of Apoptosis
  • 02:34Apoptotic Body Isolation using FACS
  • 04:22ApoBD Isolation via Differential Centrifugation
  • 06:02Flow Cytometry Gating Strategy
  • 10:56Representative Result: Validation of Apoptosis
  • 11:16Representative Result: Comparison of ApoBD-Enriched Sample Purity
  • 11:47Conclusion

Summary

Automatic Translation

Automatic Translation

A workflow using flow cytometry or differential centrifugation is developed to detect, quantify and isolate apoptotic bodies from an apoptotic sample to high purity.

Tags

Keywords: Apoptotic BodiesExtracellular VesiclesHematosisCell ClearanceIntracellular CommunicationApoptosis InductionUV IrradiationApoptotic MorphologiesApoptotic BlebbingFACSAnnexin VTOPO-3Cell StrainerCell LinesTHB1 Monocytes

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