A Cell-based Assay to Investigate Non-muscle Myosin II Contractility <em>via</em> the Folded-gastrulation Signaling Pathway in <em>Drosophila</em> S2R+ Cells

Kimberly A. Peters; Elizabeth Detmar; Liz Sepulveda; Corrina Del Valle; Ruth Valsquier; Anna Ritz; Stephen L. Rogers; Derek A. Applewhite

doi:10.3791/58325

/

A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells

A Cell-based Assay to Investigate Non-muscle Myosin II Contractility <em>via</em> the Folded-gastrulation Signaling Pathway in <em>Drosophila</em> S2R+ Cells

JoVE Journal
Developmental Biology

This content is Free Access.

JoVE Journal Developmental Biology

A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells

A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells

7,478 Views

•

07:15 min

•

August 19, 2018

DOI:

10.3791/58325-v

DOI:

10.3791/58325-v

•

07:15 min

August 19, 2018

•

7444 Views

Kimberly A. Peters¹, Elizabeth Detmar¹, Liz Sepulveda², Corrina Del Valle², Ruth Valsquier², Anna Ritz², Stephen L. Rogers^1,3,4, Derek A. Applewhite²

¹Department of Biology,The University of North Carolina at Chapel Hill ²Department of Biology,Reed College ³Carolina Center for Genome Sciences,The University of North Carolina at Chapel Hill ⁴Lineberger Comprehensive Cancer Center,The University of North Carolina at Chapel Hill

Summary

Here we describe a contractility assay using Drosophila S2R+ cells. The application of an exogenous ligand, folded gastrulation (Fog), leads to the activation of the Fog signaling pathway and cellular contractility. This assay can be used to investigate the regulation of cellular contractility proteins in the Fog signaling pathway.