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Immunology and Infection
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“Liver-on-a-Chip” Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
“Liver-on-a-Chip” Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

“Liver-on-a-Chip” Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

DOI:
10.3791/58333-v

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10:25 min

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February 19, 2019

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, , , , ,
1Section of Virology, Department of Medicine,Imperial College

Chapters

  • 00:04Title
  • 01:38Assembly and Equilibration of Plates
  • 04:36Thawing and Seeding of Hepatocytes for Monocultures
  • 07:38Results: Long-term Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
  • 09:13Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to provide a step-by-step guide to perform 3-D "liver-on-a-chip" infection experiments with the hepatitis B virus.

Tags

Keywords: Liver-on-a-chipPrimary HepatocytesKupffer CellsHepatitis B VirusViral PathogenesisInfection KineticsViral ReplicationImmune InvasionPhysiological ModelDrug TestingHepatic MicroenvironmentHepatotropic InfectionsLiver DiseasesDrug MetabolismLong-term CultureSpecialized ToolsVisual DemonstrationMembraneMicrofluidic PlatesSeedingMedium Priming

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