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Culturing and Manipulation of O9-1 Neural Crest Cells

Culturing and Manipulation of O9-1 Neural Crest Cells

Article DOI: 10.3791/58346 08:32 min October 9th, 2018
October 9th, 2018



O9-1 is a multipotent mouse neural crest cell line. Here we describe detailed step-by-step protocols for culturing O9-1 cells, differentiating O9-1 cells into specific cell types, and genetically manipulating O9-1 cells by using siRNA-mediated knockdown or CRISPR-Cas9 genome editing.



O9-1 Neural Crest Cells Cell Culture Cell Line In Vitro Studies Research Applications Basal Media Conditioned Basal Media STO Cells Basement Membrane Matrix Cell Recovery Cryo-vial
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