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Journal / Immunology and Infection / Høj overførselshastighed måling af Plasma membran …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Høj overførselshastighed måling af Plasma membran genforsegling effektivitet i pattedyrceller
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Høj overførselshastighed måling af Plasma membran genforsegling effektivitet i pattedyrceller

Article DOI: 10.3791/58351-v • 10:07 min • January 7th, 2019
January 7th, 2019 •
1,2,3, 4, 1,2,3
1Department of Microbial Infection and Immunity, The Ohio State University, 2Department of Microbiology, The Ohio State University, 3Infectious Diseases Institute, The Ohio State University, 4Division of Biostatistics, College of Public Health, The Ohio State University

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Her beskriver vi en høj overførselshastighed fluorescens-baseret analyse, der måler plasmamembran genforsegling effektivitet gennem fluorometriske og billedbehandling analyser i levende celler. Denne analyse kan anvendes til screening af narkotika eller target gener, der regulerer plasmamembran genlukning i pattedyrsceller.

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Immunologi og infektion spørgsmålet 143 Plasma membran lukning membran reparation muskelsvind propidium Iodid carbocyanine nukleinsyre bindende farvestof poreformede toksin listeriolysin O
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