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Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array (HMCA)-based Plates
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Cancer Research
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JoVE Journal Cancer Research
Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array (HMCA)-based Plates

Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array (HMCA)-based Plates

DOI:
10.3791/58359-v

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08:32 min

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October 25, 2018

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, , , , , , ,
1Physics Department,Bar-Ilan University

Chapters

  • 00:04Title
  • 01:17Hyrdogel Micro-chamber Array (HMCA) Plate Embossing
  • 02:28Cell Loading and Spheroid Formation
  • 04:26Invasion Assay Acquisition and Analysis
  • 06:06Results: Representative Influence of Extracellular Matrix (ECM) Composition on Spheroid Invasion
  • 07:25Conclusion

Summary

Automatic Translation

Automatic Translation

A HMCA-based imaging plate is presented for invasion assay performance. This plate facilitates the formation of three-dimensional (3D) tumor spheroids and the measurement of cancer cell invasion into the extracellular matrix (ECM). The invasion assay quantification is achieved by semi-automatic analysis.

Tags

Keywords: 3D Cell CultureHydrogel Micro-chamber ArrayTumor SpheroidAnti-metastatic Drug ScreeningCell InvasionPDMS StampAgarose GelUV SterilizationCell Seeding

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