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Journal / Environment / Individual Culturing of Tigriopus Copepods and…
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JoVE Journal Environment
Individual Culturing of Tigriopus Copepods and Quantitative Analysis of Their Mate-guarding Behavior
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Individual Culturing of Tigriopus Copepods and Quantitative Analysis of Their Mate-guarding Behavior

Article DOI: 10.3791/58378-v • 06:24 min • September 26th, 2018
September 26th, 2018 •
1, 1
1Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego

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Summary
Automatic Translation

Mate-guarding behavior plays an important role in reproduction of intertidal copepods of the genus Tigriopus. However, methods for studying this behavior have not been well described. Here we describe methods for: 1) individual culture of virgin Tigriopus animals, and 2) quantitative analysis of their mate-guarding behavior.

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Individual Culturing Tigriopus Copepods Quantitative Analysis Mate-guarding Behavior Culturing Technique Pairing Experience Development Tracking Behavior Analysis Method Reproductive Behavior Genetic Factors Environmental Factors Pasteur Pipette Gravid Females Culture Medium Six-well Cell Culture Plate Incubator Egg Sacs Copepodid Stage P-10 Micropipette Molted Exuviae Antenna Morphology
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