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JoVE Journal Cancer Research
Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens
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Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

Article DOI: 10.3791/58390-v • 10:49 min • January 21st, 2019
January 21st, 2019 •
1, 1, 1, 1, 2, 1, 3, 3, 1, 2, 1, 1, 4, 3, 1
1Laboratory of Pathology, MedImmune, 2Laboratory of Pathology, MedImmune, 3Akoya Biosciences Inc., 4Department of Translational Molecular Pathology, University of Texas - MD Anderson Cancer Center

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Summary
Automatic Translation

A detailed protocol for a six-marker multiplex immunofluorescence panel is optimized and performed, using an automated stainer for more consistent results and a shorter procedure time. This approach can be directly adapted by any laboratory for immuno-oncology studies.

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Automated Multiplex Immunofluorescence Panel Immuno-oncology Studies Formalin-fixed Carcinoma Tissue Specimens Multiplex Immunofluorescence Protocols Multi-spectral Immunofluorescence Immune Oncology Immune Cell Types Auto-immune Therapies Inflammatory Therapies Location And Phenotype Of Immuno Cells Tumor-specific Immunotherapy Optimization And Validation Of The Protocol Artifacts Associated With Multi-spectral Imaging Base Multi-plex Protocol Automated Stainer
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