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Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens
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Cancer Research
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JoVE Journal Cancer Research
Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

DOI:
10.3791/58390-v

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10:49 min

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January 21, 2019

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, , , , , , , , , , , , , ,
1Laboratory of Pathology,MedImmune, 2Laboratory of Pathology,MedImmune, 3Akoya Biosciences Inc., 4Department of Translational Molecular Pathology,University of Texas – MD Anderson Cancer Center

Chapters

  • 00:04Title
  • 01:17Multiplex Immunofluorescence (mIF) Protocol Program Creation
  • 03:09Reagent Preparation
  • 05:19Multispectral Imaging
  • 06:45Spectral Unmixing, Fluorescence Intensity, and Signal Attenuation
  • 08:15Results: Representative mIF Imaging
  • 09:59Conclusion

Summary

Automatic Translation

Automatic Translation

A detailed protocol for a six-marker multiplex immunofluorescence panel is optimized and performed, using an automated stainer for more consistent results and a shorter procedure time. This approach can be directly adapted by any laboratory for immuno-oncology studies.

Tags

Keywords: Multiplex ImmunofluorescenceImmuno-oncologyAutomated StainingProtocol OptimizationImmune Cell PhenotypingPrognostic And Predictive BiomarkersSpectral ImagingPeroxidase InhibitionPrimary And Secondary AntibodiesTyramide Signal AmplificationAntigen RetrievalDrop ControlsIsotype Controls

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