Biology
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Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
Chapters
Summary January 7th, 2019
Here, a protocol for the isolation and characterization of CD4+ T-cell subsets from human peripheral blood is described. Purified CD4+ T-cells are analyzed by flow cytometry to determine proportions of T-follicular helper cell subsets.
Transcript
This method can help to demonstrate the differences between circulating T-follicular helper cell subsets in healthy and diseased states.The main advantages of this technique are that it's fast, that the samples can be batch-processed, and that the cells remain alive for downstream functional analysis.This technique facilitates the evaluation of circulating T-follicular helper subsets as biomarkers for disease progression as they correlate with markers of clinical severity.Helping with the demonstration of this procedure will be Dr.Lucia Pi
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