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Journal / Medicine / Generation av Human 3D Lung vävnadskulturer…
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Medicine

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JoVE Journal Medicine
Generation av Human 3D Lung vävnadskulturer (3D-LTCs) för sjukdomen modellering
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Generation av Human 3D Lung vävnadskulturer (3D-LTCs) för sjukdomen modellering

Article DOI: 10.3791/58437-v • 05:47 min • February 12th, 2019
February 12th, 2019 •
1,2,3, 4,5,6, 4,5,6, 2,7, *1,2,3, *1,2,3,8
1Comprehensive Pneumology Center, Ludwig-Maximilians-Universität and Helmholtz Zentrum Munich, 2German Center of Lung Research (DZL), 3Translational Lung Research and CPC-M bioArchive, Helmholtz Zentrum München, Comprehensive Pneumology Center Munich DZL/CPC-M, 4Department of Experimental Medical Science, Lung Bioengineering and Regeneration, Lund University, 5Wallenberg Center for Molecular Medicine, Lund University, 6Stem Cell Centre, Lund University, 7Asklepios Fachkliniken Munich-Gauting, 8Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado
* These authors contributed equally

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Här presenterar vi ett protokoll för beredning av agaros-fyllda mänsklig precision-cut lung skivor från opererande patientens vävnad som är lämpliga för att generera 3D lung vävnadskulturer till modell mänskliga lungsjukdomar i biologisk och biomedicinsk studier.

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Medicin fråga 144 3D vävnadsodling ex-vivo vävnadsodling precision-cut lung skivor avgiften 3D-LTCs lungsjukdom sjukdom djurmodeller mänskliga ex vivo-modeller lungfibros
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