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Journal / Immunology and Infection / Immunisering af voksen zebrafisk for præklinisk…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Immunisering af voksen zebrafisk for præklinisk Screening af DNA-baserede vacciner
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Immunisering af voksen zebrafisk for præklinisk Screening af DNA-baserede vacciner

Article DOI: 10.3791/58453-v • 05:39 min • October 30th, 2018
October 30th, 2018 •
*1, *1, 1,2, 1,3,4,5
1BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, 2MedEngine Oy, 3Department of Pediatrics, Tampere University Hospital, 4Department of Children and Adolescents, Oulu University Hospital, 5PEDEGO Research Unit, Medical Research Center, University of Oulu
* These authors contributed equally

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Her beskriver vi en protokol for immunisering af de voksne zebrafisk (Danio rerio) med en DNA-baseret vaccine og demonstrere validering af en vellykket vaccination begivenhed. Denne metode er velegnet til præklinisk screening af vaccine kandidater i forskellige infektion modeller.

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Immunologi og infektion spørgsmål 140 DNA-vaccine vaccination zebrafisk fluorescens imaging pCMV-NGL plasmid microinjector intramuskulær præklinisk screening dyremodel mykobakterier antigen immunisering
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