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A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci
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A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci

A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci

DOI:
10.3791/58502-v

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05:03 min

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October 29, 2018

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, , , , , , , , , , ,
1Department of Method Development and Analytics,Agroscope, 2Swiss Tropical and Public Health Institute, 3University of Basel, 4Swiss Federal Plant Protection Service,Federal Office for Agriculture, 5OptiGene Limited, 6Fera Science Limited, 7School of Natural and Environmental Sciences,Newcastle University, 8Department of Plants and Plant Products,Agroscope

Chapters

  • 00:04Title
  • 00:41Preparing Ready-to-Use B. tabaci LAMP Kit
  • 01:39DNA Extraction and B. tabaci LAMP Assay
  • 02:58LAMP Assay Read-out
  • 03:25Results: Intercepted B. tabaci Insect Specimens Analyzed and Validated via LAMP Assay
  • 04:20Conclusion

Summary

Automatic Translation

Automatic Translation

This paper reports the protocol for a rapid identification assay for Bemisia tabaci based on loop-mediated isothermal amplification (LAMP) technology. The protocol requires minimal laboratory training and can, therefore, be implemented on-site at points of entry for plant imports such as seaports and airports.

Tags

Keywords: LAMP AssayBemisia TabaciInsect PestDNA ExtractionIsothermal AmplificationLAMP Analysis DeviceMelting Temperature AnalysisAutomated LAMP Validation

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