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JoVE Journal
Immunology and Infection

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Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
 

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Article doi: 10.3791/58509
November 26th, 2018

Summary November 26th, 2018

Here, we present an integrated protocol that measures monocyte subpopulation trafficking under flow in vitro by use of specific surface markers and confocal fluorescence microscopy. This protocol can be used to explore sequential recruitment steps as well as to profile other leukocyte subtypes using other specific surface markers.

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