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Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
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Immunology and Infection
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JoVE Journal Immunology and Infection
Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

DOI:
10.3791/58509-v

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09:16 min

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November 26, 2018

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, , , ,
1Hematology service, Centre Médical Universitaire (CMU), Medical faculty,University of Geneva, 2Department of Pathology and Immunology, Centre Médical Universitaire (CMU), Medical faculty,University of Geneva, 3Department of Cell Physiology and Metabolism, Centre Médical Universitaire (CMU), Medical faculty,University of Geneva

Chapters

  • 00:04Title
  • 00:54Human Umbilical Vein Endothelial Cell (HUVEC) Staining and Human Pan Monocyte Isolation
  • 03:20Fluidic System Preparation
  • 05:04Time-lapse Imaging of Monocyte Recruitment Under Flow and Image Analysis
  • 06:56Results: Representative Monocyte Transmigration Analysis
  • 08:25Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present an integrated protocol that measures monocyte subpopulation trafficking under flow in vitro by use of specific surface markers and confocal fluorescence microscopy. This protocol can be used to explore sequential recruitment steps as well as to profile other leukocyte subtypes using other specific surface markers.

Tags

Keywords: Monocyte SubpopulationsLeukocyte AdhesionTrans-endothelial MigrationHematopoietic CellsHUVECCMFDADensity Gradient SeparationPAN Monocyte IsolationTransmigrationAdhesionM199 MediumBSA

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