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JoVE Journal
Biochemistry

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Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag
 

Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag

Article doi: 10.3791/58532
November 1st, 2018

Summary November 1st, 2018

We here present a proven and tested protocol for the purification of chloroplast protein import complexes (TOC-TIC complex) using the TAP-tag. The one-step affinity-isolation protocol can potentially be applied to any protein and be used to identify new interaction partners by mass spectrometry.

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