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Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag

Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag

DOI:
10.3791/58532-v

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07:01 min

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November 01, 2018

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, , ,
1Laboratory of Plant Physiology,University of Neuchatel, 2Institut fur Biochemie und Biotechnologie,Martin-Luther-Universitat Halle-Wittenberg

Chapters

  • 00:04Title
  • 01:14Isolation and Solubilization of the Membrane Fraction
  • 03:27Immunoprecipitation
  • 05:03Results: Immunoblot Analysis of the Purified Protein Complex
  • 06:11Conclusion

Summary

Automatic Translation

Automatic Translation

We here present a proven and tested protocol for the purification of chloroplast protein import complexes (TOC-TIC complex) using the TAP-tag. The one-step affinity-isolation protocol can potentially be applied to any protein and be used to identify new interaction partners by mass spectrometry.

Tags

Keywords: ChloroplastTransloconProtein ComplexesTAP TagTOCTICArabidopsisAffinity PurificationMembrane FractionsDetergentSolubilizationUltracentrifugationGrinding BufferCentrifugationHomogenization

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