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Biochemistry

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Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
 

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

Article DOI: 10.3791/58542-v
January 18th, 2019

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Summary January 18th, 2019

Here we present a protocol for murine in vivo labeling of glomerular cell surface proteins with biotin. This protocol contains information on how to perfuse mouse kidneys, isolate glomeruli, and perform endogenous immunoprecipitation of the protein of interest.

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