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Isolering af Glomeruli og In Vivo mærkning af glomerulær celle overflade proteiner
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
DOI:

09:12 min

January 18, 2019

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Chapters

  • 00:04Title
  • 00:47Surgical Procedure
  • 03:00In Vivo Biotinylation and Glomeruli Isolation
  • 05:29Glomeruli Washing and Quantification
  • 06:25Results: Representative In Vivo Labeling of Glomerular Cell Surface Proteins
  • 08:31Conclusion

Summary

Automatic Translation

Her præsenterer vi en protokol for murine i vivo mærkning af glomerulær celle overflade proteiner med biotin. Denne protokol indeholder oplysninger om, hvordan du perfuse musen nyrer, isolere glomeruli og udføre endogene immunoprecipitation af protein af interesse.

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