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In Vitro Method to Control Concentrations of Halogenated Gases in Cultured Alveolar Epithelial Cells
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In Vitro Method to Control Concentrations of Halogenated Gases in Cultured Alveolar Epithelial Cells

Article DOI: 10.3791/58554-v • 04:56 min • October 23rd, 2018
October 23rd, 2018 •
1,2, 1,2, 3, 2,4, 2,4, 2,4, 2,4, 2, 2, 1,2, 1,2, 1,2, 2, 1,2
1Department of Perioperative Medicine, CHU Clermont-Ferrand, 2Centre National de la Recherche Scientifique Unité Mixte de Recherche (CNRS UMR) 6293, Institut National de la Santé et de la Recherche Médicale (INSERM) U1103, Laboratoire de Génétique, Reproduction et Développement (GReD), Université Clermont Auvergne, 3Department of Pharmacology, CHU Clermont-Ferrand, 4Nurse Anesthetist School, CHU Clermont-Ferrand

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Summary
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We describe an easy protocol specifically designed to reach precise and controlled concentrations of sevoflurane or isoflurane in vitro in order to improve our understanding of mechanisms involved in the epithelial lung injury and to test novel therapies for acute respiratory distress syndrome.

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In Vitro Method Halogenated Gases Cultured Alveolar Epithelial Cells Lung Injury And Repair Acute Respiratory Distress Syndrome Novel Therapies Precise And Controlled Concentrations Airtight Chamber Polypropylene Box Gas Air Mixture Input Pipe Gas Air Mixture Output Pipe Charcoal Filter Gas Analyzer Digital Thermometer Anesthetic Machine Circuit
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