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Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella
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Biochemistry
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JoVE Journal Biochemistry
Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella

Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella

DOI:
10.3791/58568-v

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11:31 min

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November 30, 2018

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, , , , ,
1Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, Collaborative Innovation Center of Chemical Science and Engineering, School of Pharmaceutical Science and Technology,Tianjin University, 2State Key Laboratory of Ecological Pest Control for Fujian/Taiwan Crops and Institute of Applied Ecology,Fujian Agriculture and Forestry University, 3Joint International Research Laboratory of Ecological Pest Control,Ministry of Education, Fuzhou, 4Fujian-Taiwan Joint Centre for Ecological Control of Crop Pests,Fujian Agriculture and Forestry University

Chapters

  • 00:04Title
  • 01:01Ligation-independent Cloning (LIC)
  • 02:38E. coli Transformation and Protein Expression
  • 04:58Fusion Protein Purification, Tag-cleaving, and Target Protein Re-purification
  • 06:12Protein Crystallization, Crystal Mounting, and Structure Determination
  • 09:56Results: Representative N-terminal Domain (NTD) Crystal Structure Determination
  • 10:53Conclusion

Summary

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Automatic Translation

In this article, we describe the protocols of protein expression, purification, crystallization and structure determination of the N-terminal domain of ryanodine receptor from diamondback moth (Plutella xylostella).

Tags

Keywords: Ryanodine ReceptorCrystal StructureInsecticideInsectProtein StructureX-ray CrystallographyProtein CrystallographyPolymerase Chain ReactionDNA AmplificationLIC VectorT4 DNA PolymeraseLigation-independent CloningBL21(DE3) E. ColiProtein Expression

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