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Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

DOI:
10.3791/58589-v

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05:30 min

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January 23, 2019

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, , , ,
1bisy e.U., 2Institute of Molecular Biotechnology,Technical University of Graz

Chapters

  • 00:04Title
  • 00:43Main Culture Cultivation and Harvesting
  • 03:30Results: Representative De-Repressed Protein Expression Analysis
  • 04:32Conclusion

Summary

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This method for the first time describes reliable experimental procedures for efficient inducible methanol-free recombinant protein production in Pichia pastoris (Komagataella phaffii), employing the carbon source regulated promoters PDCand PDF in small scale experiments while cultivation parameters can be monitored online, and a constant glycerol feed is applied.

Tags

Pichia PastorisMethanolDe-repressionRecombinant Protein ExpressionShake Flask CultivationOnline MonitoringBiomass MeasurementBluetoothCell DensityGlycerol FeedExponential Growth Phase

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