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JoVE Journal
Bioengineering

This content is Open Access.

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
 

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

Article doi: 10.3791/58589
January 23rd, 2019

Summary January 23rd, 2019

This method for the first time describes reliable experimental procedures for efficient inducible methanol-free recombinant protein production in Pichia pastoris (Komagataella phaffii), employing the carbon source regulated promoters PDCand PDF in small scale experiments while cultivation parameters can be monitored online, and a constant glycerol feed is applied.

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