Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Environment / Rilevamento di Tilapia Lago Virus mediante RT-PCR …
JoVE Journal
Environment
Author Produced

This content is Open Access.

JoVE Journal Environment
Rilevamento di Tilapia Lago Virus mediante RT-PCR convenzionale e SYBR Green RT-qPCR
Optimized Procedure for Determining the Adsorption of Phosphonates onto Granular Ferric Hydroxide using a Miniaturized Phosphorus Determination Method
Journal (Environment)
Optimized Procedure for Determining the Adsorption of Phosphonates onto Granular Ferric Hydroxide using a Miniaturized Phosphorus Determination Method
Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica
Journal (Environment)
Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica
 
Click here for the English version

Rilevamento di Tilapia Lago Virus mediante RT-PCR convenzionale e SYBR Green RT-qPCR

Article DOI: 10.3791/58596-v • 10:55 min • November 10th, 2018
November 10th, 2018 •
1, 2, 2
1Institute of Veterinary Bacteriology Vetsuisse Faculty, University of Bern, 2Department of Veterinary Microbiology and Immunology and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Questo protocollo diagnosi Tilapia Lago Virus (TiLV) nei tessuti di tilapia utilizzando metodologie di RT-PCR. L'intero metodo è descritto da dissezione del tessuto per estrazione di RNA totale, seguita dalla sintesi del cDNA e rilevamento di TiLV da PCR convenzionale o PCR quantitativa utilizzando dsDNA vincolante una tintura fluorescente legante.

Tags

Scienze ambientali problema 141 Tilapia Lago virus tilapia diagnosi RT-PCR RT-qPCR orthomyxo-come il virus
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter