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Journal / Biochemistry / A Rat Methyl-Seq Platform to Identify Epigenetic…
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Biochemistry

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JoVE Journal Biochemistry
A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure
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A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure

Article DOI: 10.3791/58617-v • 09:06 min • October 24th, 2018
October 24th, 2018 •
1, 1, 1, 1, 1, 1,3, 1,2, 1
1Departments of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, 2Department of Medicine, Johns Hopkins School of Medicine, 3Department of Mental Health, Johns Hopkins School of Public Health

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Summary
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Here, we describe the protocol and implementation of Methyl-Seq, an epigenomic platform, using a rat model to identify epigenetic changes associated with chronic stress exposure. Results demonstrate that the rat Methyl-Seq platform is capable of detecting methylation differences that arise from stress exposure in rats.

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Rat Methyl-Seq Platform Epigenetic Changes Stress Exposure DNA Methylation Sequencing-based Tool Environmental Factors Array-based Platforms Physiological Processes Organism Sequence Data Stress Impact On DNA Methylation Environmental Toxicants High-fat Diet Diabetes Cardiovascular Disease DNA Shearing Bioanalyzer DNA-binding Magnetic Beads Ethanol Heat Block Nuclease-free Water
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