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Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques
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Bioengineering
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JoVE Journal Bioengineering
Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques

Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques

DOI:
10.3791/58619-v

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08:28 min

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November 02, 2018

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, ,
1Department of Biomedical Engineering,Duke University

Chapters

  • 00:04Title
  • 00:43Generate Samples for Imaging
  • 02:13Set up Microscope for Imaging
  • 03:57Acquire FRET-FRAP Data
  • 06:53Results: Vinculin Force-Sensitive Dynamics at the Focal Adhesion
  • 07:52Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for the simultaneous use of Förster resonance energy transfer-based tension sensors to measure protein load and fluorescence recovery after photobleaching to measure protein dynamics enabling the measurement of force-sensitive protein dynamics within living cells.

Tags

Keywords: Force-sensitive Protein DynamicsFluorescent TechniquesMechanobiologyVinculinTalinCadherinsNesprinTension Sensor ConstructFibronectinFRAP LaserAcceptor FluorophoreMechanically-sensitive ProteinsCell-cell InteractionsCell-environment InteractionsMolecular ScaleLiving Cells

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