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Journal / Immunology and Infection / Digestion du foie murin pour une cytométrie de…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Digestion du foie murin pour une cytométrie de cellules endothéliales lymphatiques
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Digestion du foie murin pour une cytométrie de cellules endothéliales lymphatiques

Article DOI: 10.3791/58621-v • 08:07 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1,2
1Division of Gastroenterology and Hepatology, Department of Medicine, University of Colorado Anschutz Medical Campus, School of Medicine, 2Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus

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Ce protocole vise à identifier les populations de cellules endothéliales lymphatiques dans le foie à l’aide de marqueurs décrits. Nous utilisons la collagénase IV et DNase et un hachage doux de tissu, combiné à la cytométrie en flux, afin d’identifier une population distincte de cellules endothéliales lymphatiques.

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Immunologie et Infection numéro 143 foie cellules endothéliales lymphatiques cytométrie en flux cellules endothéliales sinusoïdales hépatiques collagénase IV
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