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Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System
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JoVE Journal Bioengineering
Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System

DOI:
10.3791/58624-v

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07:44 min

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October 20, 2018

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, , , , , , ,
1Key Laboratory for Regenerative Medicine, Ministry of Education,Jinan University, 2Department of Developmental and Regenerative Biology,Jinan University, 3Nansha College Preparatory Academy, 4Department of Cardiology,First Affiliated Hospital of Jinan University

Chapters

  • 00:00Title
  • 01:02Preparations Before Cell Sheet Construction
  • 01:39Preparing Cells for Cell Sheet Construction
  • 03:22Preparing BMSCs and RAD16-I Peptide Hydrogel Mixture
  • 04:36In vitro Culture of 3D Multilayered Cell Sheet Using a Dynamic Culture System
  • 05:18Obtain the Multilayered MSC Cell Sheet
  • 05:49Results
  • 06:34Conclusion

Summary

Automatic Translation

Automatic Translation

This article provides an efficient and feasible method for constructing multilayered stem cell sheets with favorable stem cell property.

Tags

Keywords: Mesenchymal Stem CellCell Sheet3D Dynamic Culture SystemMultilayered Stem Cell SheetPericardium ScaffoldNonapeptide HydrogelCell Nutrition SupplyCell RetentionCell Survival

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